Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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391 – 405 of 3,516 results

391

HEPES, ≥99% (Dry Basis), Ultrapure

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-[2-Hydroxyethyl]piperazine-N'-[2-Ethanesulfonic acid] PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

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Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-[2-Hydroxyethyl]piperazine-N'-[2-Ethanesulfonic acid]
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

392

Tricine-SDS Sample Buffer (2X), non-reducing, Thermo Scientific™

Pricing & Availability
Specifications

Name Note	non-reducing
Health Hazard 3	GHS P Statement P280-P264-P305+P351+P338-P337+P313 Wear protective gloves/protective clothing/eye protection/face protection. Wash thoroughly after handling. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention.
Health Hazard 2	GHS H Statement H319 Causes serious eye irritation.
Color	Blue
Physical Form	Liquid
Health Hazard 1	H335
Chemical Name or Material	Tricine-SDS Sample Buffer
TSCA	Yes
Recommended Storage	Ambient temperatures
Concentration	2X

393

Thermo Scientific Chemicals Hanks' Balanced Salt Solution (10X), with calcium, with magnesium, with phenol red

Pricing & Availability
Specifications

Color	Red
Physical Form	Liquid
Recommended Storage	Ambient temperatures

394

BES, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

395

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Thermo Scientific Chemicals MES, 99+%, <1% water

CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00006181 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O

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Specifications

PubChem CID	78165
CAS	4432-31-9
Molecular Weight (g/mol)	195.233
ChEBI	CHEBI:39005
MDL Number	MFCD00006181
SMILES	C1COCCN1CCS(=O)(=O)O
Synonym	2-(N-Morpholino)ethanesulfonic acid
IUPAC Name	2-morpholin-4-ylethanesulfonic acid
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Molecular Formula	C6H13NO4S

396

Thermo Scientific Chemicals CAPS, 0.5M buffer soln., pH 10.5

CAS: 1135-40-6 Molecular Formula: C9H19NO3S Molecular Weight (g/mol): 221.32 MDL Number: MFCD00003837 InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid SMILES: OS(=O)(=O)CCCNC1CCCCC1

Pricing & Availability
Specifications

CAS	1135-40-6
Molecular Weight (g/mol)	221.32
MDL Number	MFCD00003837
SMILES	OS(=O)(=O)CCCNC1CCCCC1
IUPAC Name	3-(cyclohexylamino)propane-1-sulfonic acid
InChI Key	PJWWRFATQTVXHA-UHFFFAOYSA-N
Molecular Formula	C9H19NO3S

397

BES, 99+%, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

Pricing & Availability
Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

398

N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid, free acid, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

Pricing & Availability
Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	N, N-Bis(2-hydroxyethyl)taurine,N, N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

399

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(Ethylenedinitrilo)tetraacetic Acid, Powder, Spectrum™ Chemical

CAS: 60-00-4

Pricing & Availability
Specifications

CAS	60-00-4

400

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 8.0

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

401

MP Biomedicals™ Tris-Glycine Running Buffer for Native PAGE

Powder buffer salts for making Native-PAGE buffer.

Pricing & Availability

402

Transformation buffer-2, pH 7.5, Thermo Scientific™

For transformation of competent cells

Pricing & Availability
Specifications

Physical Form	Liquid
pH	7.5
Recommended Storage	Keep cold

403

MilliporeSigma™ Calbiochem™ OmniPur™ 10X TAE Buffer, Liquid Concentrate

For use in molecular biology applications

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404

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Thermo Scientific Chemicals Bicine, 99+%, for biochemistry

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	N, N-Bis(2-hydroxyethyl)glycine,Diethylolglycine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

405

Thermo Scientific Chemicals Hanks' Balanced Salt Solution (10X), with calcium, with magnesium, without phenol red

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Recommended Storage	Ambient temperatures

Life Science Buffers

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(Ethylenedinitrilo)tetraacetic Acid, Powder, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

(Ethylenedinitrilo)tetraacetic Acid, Powder, Spectrum™ Chemical